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Moderate-intensity immunostaining of sparsed axons in the neocortex and dense axonal networks in some distinct brain areas like nucleus accumbens, anterior hypothalamus, BNST, hypothalamic paraventricular nucleus, thalamic paraventricular nucleus, arcuate nucleus, amygdala nuclei, piriform cortex, periaqueductal gray matter, locus coeruleus, parabrachial nucleus and some distinct medullar nuclei, like n. tractus solitarii and dorsal motor nucleus of the vagus are noticed.
In addition, sparse neuronal cell bodies are noticed in the piriform cortex and more in the central amygdala nuclei.
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Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.